Understanding and intervening at the connection between group dynamics and member behavior has been a long-standing challenge. Functional subgrouping (FS) is the core Systems-centered training and therapy (SCT) method and is hypothesized to solve vexing member/group dynamic problems such as scapegoating. The self-report Functional Subgrouping Questionnaire-2 (FSQ-2) was developed to measure the amount of functional subgrouping in a group. The current FSQ-2 construct validity study aims to determine whether: 1) FSQ-2 scores change across sessions; 2) level of SCT experience is related to scores; and 3) SCT experience moderates change in FSQ-2 scores over SCT training. Results from groups at various SCT training events showed higher FSQ-2 responses for those with greater SCT experience, and increased scores over time. However, change over time on most FSQ-2 items did not depend on SCT experience level. Findings provide further support for the construct validity of the FSQ-2, and thus also for the SCT model. Suggestions for future SCT/FSQ-2 research are made.